Enhancement of protozoan pathogen Perkinsus marinus infections in American oysters Crassostrea virginica exposed to the chemical carcinogen n-nitrosodiethylamine (DENA)

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Suppression of chemiluminescence of eastern oyster (Crassostrea virginica) hemocytes by the protozoan parasite Perkinsus marinus.

Experiments were conducted to determine the ability of the protistan parasite, Perkinsus marinus, to inhibit chemiluminescence of hemocytes from the eastern oyster, Crassostrea virginica. Luminol-enhanced chemiluminescence (CL) was used to measure the production of reactive oxygen intermediates (ROI) generated by oyster hemocytes using zymosan as a stimulant. To determine whether P. marinus sup...

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Discovery of genes expressed in response to Perkinsus marinus challenge in Eastern (Crassostrea virginica) and Pacific (C. gigas) oysters.

The protozoan pathogen Perkinsus marinus is the causative agent of Dermo, a lethal disease of the eastern oyster Crassostrea virginica, but not the Pacific oyster Crassostrea gigas. To understand the response of these two oysters to parasite exposure, a suppression subtractive hybridization (SSH) method was employed to characterize genes up-regulated during parasite challenge in both hemocytes ...

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Acid-Base Status of the Oyster Crassostrea virginica in Response to Air Exposure and to Infections by Perkinsus marinus.

Hemolymph acid-base variables were investigated in the Eastern oyster, Crassostrea virginica, to determine its responses to air exposure and to infections by the parasite Perkinsus marinus. Infected and uninfected oysters were subjected to two treatments of temperature (21° and 30°C) and air exposure (5 and 24 h). Upon exposure to air, oysters underwent a respiratory acidosis that remained unco...

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Perkinsus marinus tissue distribution and seasonal variation in oysters Crassostrea virginica from Florida, Virginia and New York.

Perkinsus marinus infection intensity was measured in eastern oysters Crassostrea virginica collected in October and December 1993, and March, May, and July 1994 from 3 U.S. sites: Apalachicola Bay (FL), Chesapeake Bay (VA), and Oyster Bay (NY). Gill, mantle, digestive gland, adductor muscle, hemolymph, and remaining tissue (including gonadal material and rectum) were dissected from 20 oysters ...

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Pallial mucus of the oyster Crassostrea virginica regulates the expression of putative virulence genes of its pathogen Perkinsus marinus.

Perkinsus marinus is a pathogen responsible for severe mortalities of the eastern oyster Crassostrea virginica along the East and Gulf coasts of the United States. When cultivated, the pathogenicity of this microorganism decreases significantly, hampering the study of its virulence factors. Recent investigations have shown a significant increase of the in vivo virulence of P. marinus exposed to...

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ژورنال

عنوان ژورنال: Diseases of Aquatic Organisms

سال: 1988

ISSN: 0177-5103,1616-1580

DOI: 10.3354/dao005205